JMM secretary-general Tengku Amalluddin Sani Raja Ahmad Sufian urged Ambiga to come clean about her actual beliefs and why it merited such a stand.
He said it was important to understand Ambiga's stand as she was a prominent figure of society.
"What belief drives her (Ambiga) to advocate LGBT so seriously?
"As far as we know, divine rights are paramount and far above human rights. This is the basis of every government administration and the law," he said when contacted yesterday.
He also said that the laws of any country were not inherited on the basis of human logic.
Amalluddin added that Ambiga had a duty to declare her religious faith so that the public would be able to understand the connection between her beliefs and her struggle for LGBT rights.
Ambiga has been under fire regarding LGBT rights since last year, when it was reported that she would officiate a festival organised by Seksualiti Merdeka, a movement championing freedom of sexual orientation and identity.
The festival was subsequently banned after police reports were lodged against it.
Prior to the Bersih rally this year, former Pas deputy president Nasharuddin Mat Isa also labelled Ambiga "unfit" to lead Bersih and a "threat to society" for supporting homosexuality.
Meanwhile, when contacted yesterday, Ambiga refused to comment on the matter.